KAIROS Staff Publications

Selected KAIROS Scientific Staff Publications

Solid-phase high-throughput screening of enzyme variants: Detecting nitrilase activity. Industrial Biotechnology 2:102-105, 2005.

Combinatorial Mutagenesis Algorithms, Digital Imaging Spectroscopy, and Solid-Phase Assays for Directed Evolution. In, Enzyme Functionality, Design, Engineering, and Screening. Ed. A. Svendsen. Marcel Dekker, Inc., New York. 2004

Evolving and Screening Enzymes for New Activities on Polymer Substrates. In, Biocatalysis in Polymer Science. Eds: R.A. Gross and H.N.Cheng. American Chemical Society, Washington D.C. 2003

Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase. Protein Engineering, 2001.

Complex Microbial Communities Inhabiting Sulfide-rich Black Mud from Marine Coastal Environments. Biotechnology et alia, <www.et-al.com>8:1-16, 2000.

Solid Phase Enzyme Screening. ASM News, 2000.

Molecular Phylogenetic Evidence for Noninvasive Zoonotic Transmission of Staphylococcus intermedius from a Canine Pet to a Healthy Human Host. Journal of Clinical Microbiology, 2000.

Directed Evolution and Solid Phase Enzyme Screening. SPIE, 3926:186-191 Biotechnology et alia, <www.et-al.com>7:1-6, 2000.

Multispectral Bacterial Identification. SPIE, 3913: 45-53. Biotechnology et alia, <www.et-al.com>6:1-9, 2000.

Graphical User Interface for Single-Pixel Spectroscopy. SPIE, 3924:108-115. Biotechnology et alia, <www.et-al.com>5:1-8, 2000.

Hydrogen Bonding and Circular Dichroism of Bacteriochlorophylls in the Rhodobacter capsulatus Light Harvesting 2 Complex Altered by Combinatorial Mutagenesis. Biochemistry, 1998.

Dramatic Reduction in Fluorescence Quantum Yield in Mutants of Green Fluorescent Protein due to Fast Internal Conversion. Chemical Physics, 1998.

Calibration of Fluorescence Resonance Energy Transfer in Microscopy Using Genetically Engineered GFP Derivatives on Nickel Chelating Beads. Biotechnology et alia, <www.et-al.com>3:1-18, 1997.

Fluorescence Imaging MicroSpectrophotometer (FIMS). Biotechnology et alia, <www.et-al.com>1:1-16, 1997.

Site-Directed Mutations Near the L-Subunit D-Helix of the Purple Bacterial Reaction Center: A Partial Model for the Primary Donor of Photosystem II. Biochemistry, 1997.

Time-Resolved Spectroscopy of Wild-type and Mutant Green Fluorescent Proteins Reveals Excited State Deprotonation Consistent with Fluorophore-Protein Interactions. Chemical Physics, 1996.

Structure and Fluorescence Mechanism of GFP. Nature Biotechnology, 1996.

Comparison of a b-glucosidase and a b-mannosidase from the Hyperthermophilic Archeon Pyrococcus furiosus: Purification, Characterization, Gene Cloning and Sequence Analysis. J. Biol. Chem. 271, 1996.

Dual Color Microscopic Imagery of Cells Expressing the Green Fluorescent Protein and a Red-Shifted Variant. Gene, 1996.

Fluorescent Proteins and Applications. Vol. 173, dedicated issue of Gene.

Fluorescence Resonance Energy Transfer Between Blue Emitting and Red-shifted Excitation Derivatives of the Green Fluorescent Protein. Gene, 1996.

Searching Sequence Space. Nature Biotechnology, 1995.

Green Fluorescent Pets. Science, 1995.

Red-Shifted Excitation Mutants of the Green Fluorescent Protein. Nature Biotechnology, 1995.

Context Dependence of Phenotype Prediction and Diversity in Combinatorial Mutagenesis. Protein Engineering, 1995.

Characterization of DNA Restriction-modification Systems in Spirulina platensis Strain Pacifica. J. Appl. Phycology, 1995.

Estimating Protein Function from Combinatorial Sequence Data Using Decision Algorithms and Neural Networks. Drug Dev. Res, 1994.

Digital Imaging Spectroscopy for Massively Parallel Screening of Mutants. Meth. Enzym, 1994.

Imaging Sequence Space. Nature, 1994.

Atavistic Reaction Centre, Nature, 1993.

Searching Sequence Space: Exponential Ensemble Mutagenesis, Nature Biotechnology, 1993.

Hydropathy and Molar Volume Constraints on Combinatorial Mutants of the Photosynthetic Reaction Center. J. Mol. Biol, 1993.

Genetic Algorithms and Recursive Ensemble Mutagenesis in Protein Engineering. Complexity International. http://www.csu.edu.au/ci/vol1/fuellen/REM.html

Recursive Ensemble Mutagenesis. Protein Engineering, 1993.

Study of Wild Type and Genetically Modified Reaction Centers from Rhodobacter capsulatus: Structural Comparison with Rhodopseudomonas viridis and Rhodobacter sphaeroides. Biophys. J., 1993.

An Algorithmically Optimized Combinatorial Library Screened by Digital Imaging Spectroscopy. Nature Biotechnology, 1992.

An Algorithm for Protein Engineering: Simulation of Recursive Ensemble Mutagenesis. PNAS, 1992.

Optimizing Nucleotide Mixtures to Encode Specific Subsets of Amino Acids for Semi-Random Mutagenesis. Nature Biotechnology, 1992.

Analysis of Spontaneous Herbicide Resistant Revertants Derived from Rhodobacter capsulatus in which Serine L223 of the Reaction Center is Replaced with Alanine. In: Research in Photosynthesis, 1992.

Direct Observation of Vibrational Coherence in Bacterial Reaction Centers Using Femtosecond Spectroscopy. PNAS, 1991.

Reaction Centers: Interfacing Molecular Genetics and Optical Spectroscopy. Trends in Biochemical Sciences, 1991.

Genetic Coding Algorithms for Engineering Membrane Proteins. In: Reaction Centers of Photosynthetic Bacteria, 1990.

Partial Symmetrization of the Photosynthetic Reaction Center. Science, 1990.

EPR Characterization of Genetically Modified Reaction Centers of Rhodobacter capsulatus. Biochemistry, 1990.

Spectroscopic Analysis of Genetically Modified Photosynthetic Reaction Centers. Ann. Rev. of Biophysics and Biophysical Chemistry, 1990.