MicroDIS

Also known as HIRIM, this instrument is based on a brightfield microscope and utilizes a cooled CCD camera and a computer-interfaced monochromator. Windows based software controls data acquisition, processing and display such that the ground state absorption spectrum of every pixel in a field of view can be determined simultaneously. This software also includes tools for feature extraction and sorting of spectra by various criteria which can be visualized through the use of color contour plots. Preliminary data acquired with a prototype of MicroDIS include spectra and images of H&E stained thin sections and naturally pigmented algal samples. An HTML copy of this manuscript, which is readable by almost all browsers, can be found on this web site, and a reprint of a published manuscript is available to browsers which support MS Word or PDF.

Single pixel absorption spectra from a slide stained with hematoxylin and eosin (H&E). The image window shows a transverse section of cardiac tissue back-painted based on the sorted single-pixel spectra. Clear areas in the slide are sorted to the bottom of the contour plot and pseudocolored green. Pixels indicating hematoxylin absorption (610 nm) are painted purple and coincide with nuclei. Eosinophilic tissue is painted red. Derivative plots of this H&E stained tissue can also be displayed in the contour plot.

MicroDIS patents are issued and pending.

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