Method in Enzymology 1994 (246):732-48
Youvan, DC., Goldman, E., Delagrave, S., and Yang, M.M.
Palo Alto Institute of Molecular Medicine, 2462 Wyandotte Street, Mountain View, CA 94043.
Massachusetts Institute of Technology, Department of Chemistry, Cambridge 02139.
New combinatorial mutagenesis methods in molecular genetics make it possible to simultaneously generate million of altered proteins in a single library of mutants. In the study of pigment-protein complexes, this advance in biology necessitated invention of an instrument capable of recording spectra in a massively parallel fashion, much like the human eye. In contrast to the trichromatic eye, such an instrument should be quantitative, cover a wider range of wavelengths, and have a large number of spectral bands.
Imaging spectroscopy is defined as the coprocessing of both spatial and spectral information. Such data are usually three dimensional, consisting of two spatial dimensions (i.e, the x and y coordinates of picture elements within a scene) correlated with one dimension of spectral information (e.g., the absorption spectrum of each pixel). A monochromator can be considered as an analog imaging spectrophotometer if the entrance slit is physically moved over a scene to generate the second spatial dimension. Later, this time course of convoluted data can be used to rebuild images containing spectral data. The scanning swath of a monochromator has been employed in satellite imagery and remote sensing.
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