Reaction Centers of Photosynthetic Bacteria (1990)Michel-Beyerle M. ed. pp. 209-218, Springer-Verlag, Berlin.

 

Genetic Coding Algorithms for Engineering Membrane Proteins

Yang M.M., Coleman, W.J., & Youvan, D.C.

Massachusetts Institute of Technology, Department of Chemistry, Cambridge 02139

A solution to the problem of relating the physico-chemical properties of the amino acids to their codon sequences has been achieved by treating the genetic code as a system of linear equations and applying the numerical method, Singular Value Decomposition (SVD).  For example, hydropathy and molar volume, which are important deteminants of protein structure and function, can be quantitatively related to the nucleotide sequence. The 20 hydropathy values of the amino acid residues were remapped to 12 nucleotide-determined values which, in turn, were used to predict structural aspects the photosynthetic reaction center protein, without DNA -> protein translation.  These algorithms establish a theoretical basis for manipulating the properties of ensembles of proteins at the DNA level, which is important for engineering and analyzing combinatorial cassette libraries, and for designing reduced information content (RIC) proteins.

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