Rhodobacter capsulatus spectra from MacroDIS

Individual spectra of Rhodobacter capsulatus colonies.  Spectra were exported from the DIS analysis of the previous page.  Spectral bands in the 400 - 500nm range are due to carotenoids, while bands in the near infrared (700 - 900nm) are due to the interaction of bacteriochlorophyll and bacteriopheophytin with protein complexes. The nine strains include, plot A: bchE (Mg-protoporphyrin monomethyl ester), plot B: U43aa28E (expressing only the photosynthetic reaction center), plot C: bchL (MV-protochlorophyllide), plot D: U43L228BamHI (lacking the peripheral light harvesting antenna), plot E: bchA (2-hydroxyethyl chlorophyllide a), plot F: crtD (neurosporene), plot G: Y142 (lacking the core light harvesting antenna and reaction center), plot H: SB1003 (wild-type), and plot I: crtI (lacking phytoene dehydrogenase).

Spectra of nine different mutants sorted by the KAIROS software. The contour plot on the left shows the unsorted spectra of R. capsulatus colonies in an "absolute" absorbance mode, wherein the pseudocoloring scheme is applied to the highest and lowest values in the entire plot. The center contour plot shows the results of stretching each spectra into a "full scale" mode where each row or colony is extended over the entire range given in the contour plot's color bar in the MacroDIS graphical user interface. The contour plot on the right shows the colony spectra after application of a similarity sorting algorithm. Nine different mutant types are identified and labeled A through I; exemplary spectra were exported and graphed in the panel above.

Home l Products and Services l Contract Research and Development l Publications l Company Information  CyberDope l Employment Opportunities l Search l Contact l Copyright