Action Potential

For a long time, the process of communication between nerves and their target tissues was largely unknown to physiologists. With the development of electrophysiology and the discovery of the electrical activity of neurons, it was discovered that signal transmission from neurons to their target tissues is mediated by action potentials. An action potential is defined as a sudden, rapid, transient, and propagating change in the resting membrane potential. Only neurons and muscle cells are capable of generating an action potential; that property is called excitability.

Definition

Action potentials are nerve signals. Neurons generate and conduct these signals throughout their processes to transmit them to target tissues. Upon stimulation, they will be stimulated, inhibited, or modulated in some way.

Steps

But what causes the action potential? From an electrical aspect, it is caused by a stimulus with a certain value expressed in millivolts [mV]. Not all stimuli can cause an action potential. The appropriate stimulus must have sufficient electrical value to reduce the negativity of the nerve cell to the threshold of the action potential. Thus, there are subthreshold, threshold and suprathreshold stimuli.

  • Subthreshold stimuli cannot cause an action potential.
  • Threshold stimuli have enough energy or potential to produce an action potential (nerve impulse).
  • Suprathreshold stimuli also produce an action potential, but its strength is greater than that of threshold stimuli.
  • So, an action potential is generated when a stimulus changes the membrane potential to threshold potential values. The threshold potential is usually between -50 and -55 mV. It is important to know that the action potential behaves according to the law of all or nothing. This means that any subthreshold stimulus will not cause anything, whereas threshold and suprathreshold stimuli produce a full response from the excitable cell.

Is an action potential different depending on whether it is caused by a threshold or suprathreshold potential? The answer is no. The length and amplitude of an action potential are always the same. However, increasing the strength of the stimulus causes an increase in the frequency of an action potential. An action potential propagates along with the nerve fibre without diminishing or weakening its amplitude and length. Also, after an action potential is generated, neurons become refractory to stimuli for a certain period of time where they cannot generate another action potential.

Stages

From the point of view of ions, an action potential is caused by temporary changes in the permeability of the membrane for diffusible ions. These changes cause the ion channels to open and the ions to decrease their concentration gradients. The value of the threshold potential depends on the permeability of the membrane, the intracellular and extracellular concentration of ions, and the properties of the cell membrane.

An action potential has three phases: depolarization, overshoot, and repolarization. There are two more states of the membrane potential related to the action potential. The first is hyperpolarization that precedes depolarization, while the second is hyperpolarization that follows repolarization.

Hyperpolarization is the initial increase in membrane potential to the value of the threshold potential. The threshold potential opens voltage-gated sodium channels and causes a large influx of sodium ions. This phase is called depolarization. During depolarization, the interior of the cell becomes increasingly electropositive, until the potential approaches the electrochemical equilibrium for sodium of +61 mV. This phase of extreme positivity is the overdrive phase.

After the overshoot, the permeability to sodium suddenly decreases due to the closure of its channels. The overshoot value of the cell potential opens voltage-gated potassium channels, causing a large outflow of potassium, which lowers the electropositivity of the cell. This phase is the repolarization phase, the purpose of which is to restore the resting membrane potential. Repolarization always leads first to hyperpolarization, a state in which the membrane potential is more negative than the default membrane potential. But soon after, the membrane re-establishes the values ​​of the membrane potential.

After reviewing the functions of the ions, we can now define threshold potential more precisely as the value of the membrane potential at which voltage-gated sodium channels open. In excitable tissues, the threshold potential is about 10 to 15 mV lower than the resting membrane potential.

Refractory period

The refractory period is the time after an action potential is generated, during which the excitable cell cannot produce another action potential. There are two subphases of this period, absolute and relative refractoriness.

1. Absolute refractoriness overlaps depolarization and about 2/3 of the repolarization phase. A new action potential cannot be generated during depolarization because all voltage-gated sodium channels are already open or are opening at their maximum rate. During early repolarization, a new action potential is impossible since the sodium channels are inactive and need the resting potential to be in a closed state, from which they can return to an open state. Absolute refractoriness ends when enough sodium channels recover from their inactive state.

2. Relative refractoriness is the period in which the generation of a new action potential is possible, but only in response to a suprathreshold stimulus. This period overlaps the final 1/3 of repolarization.

Propagation of the action potential.

An action potential is generated in the body of the neuron and propagates down its axon. Propagation does not diminish or affect the quality of the action potential in any way, so the target tissue receives the same impulse no matter how far away it is from the cell body.

The action potential is generated at a point on the cell membrane. It spreads across the membrane, and each succeeding part of the membrane depolarizes sequentially. This means that the action potential does not move but instead elicits a new action potential from the adjacent segment of the neuronal membrane.

We need to emphasize that the action potential always propagates forward, never backward. This is due to the refractoriness of the parts of the membrane that were already depolarized, so the only possible direction of propagation is forward. Because of this, an action potential always propagates from the cell body, down the axon to the target tissue.

The speed of propagation depends largely on the thickness of the axon and whether or not it is myelinated. The larger the diameter, the higher the rate of propagation. Propagation is also faster if an axon is myelinated. Myelin increases the speed of propagation because it increases the thickness of the fibre. Furthermore, myelin allows for saltatory conduction of the action potential, since only the nodes of Ranvier are depolarized and myelin nodes are skipped. In unmyelinated fibres, each part of the axonal membrane must undergo depolarization, which makes propagation significantly slower.

Synapse

A synapse is a junction between the nerve cell and its target tissue. In humans, synapses are chemical, meaning that the nerve impulse is transmitted from the axon terminal to the target tissue by chemicals called neurotransmitters (ligands). If a neurotransmitter stimulates the action of the target cell, then it is an excitatory neurotransmitter. On the other hand, if it inhibits the target cell, it is an inhibitory neurotransmitter. Depending on the type of target tissue, there are central and peripheral synapses. Central synapses occur between two neurons in the central nervous system, while peripheral synapses occur between a neuron and muscle fibre, peripheral nerve, or gland.

Each synapse consists of:

  • Presynaptic membrane: membrane of the terminal button of the nerve fibre.
  • Postsynaptic membrane: membrane of the target cell.
  • Synaptic cleft: a gap between the presynaptic and postsynaptic membranes

Numerous vesicles containing neurotransmitters are produced and stored within the terminal button of the nerve fibre. When the presynaptic membrane is depolarized by an action potential, voltage-gated calcium channels open. This leads to an influx of calcium, which changes the state of certain membrane proteins in the presynaptic membrane and results in the exocytosis of the neurotransmitter in the synaptic cleft.

The postsynaptic membrane contains receptors for neurotransmitters. Once the neurotransmitter binds to the receptor, ligand-gated channels in the postsynaptic membrane open or close. These ligand-gated channels are the ion channels and their opening or closing will cause a redistribution of ions in the postsynaptic cell. Depending on whether the neurotransmitter is excitatory or inhibitory, this will result in different responses.

Biochemistry

Biochemistry, study of the chemical substances and processes that occur in plants, animals, and microorganisms and the changes they undergo during development and life. It deals with the chemistry of life, and as such draws on the techniques of analytical, organic, and physical chemistry, as well as those of physiologists interested in the molecular basis of life processes. All chemical changes within the organism, whether it be the breakdown of substances, usually to obtain the necessary energy or the accumulation of complex molecules necessary for life processes, are collectively called metabolism.

These chemical changes depend on the action of organic catalysts known as enzymes, and enzymes, in turn, depend on their existence on the genetic apparatus of the cell. It is not surprising, therefore, that biochemistry enters the investigation of chemical changes in disease, drug action, and other aspects of medicine, as well as nutrition, genetics, and agriculture.

The term biochemistry is synonymous with two somewhat older terms: physiological chemistry and biological chemistry. Aspects of biochemistry that deal with the chemistry and function of very large molecules (eg, proteins and nucleic acids) are often grouped under the term molecular biology. Biochemistry is a young science, known under that term only since around 1900. However, its origins go back much further; its early history is part of the early history of both physiology and chemistry.

Study areas

A description of life at the molecular level includes a description of all the complexly interrelated chemical changes that occur within the cell, that is, the processes known as intermediary metabolism. The processes of growth, reproduction, and heredity, also subjects of biochemists’ curiosity, are intimately related to intermediary metabolism and cannot be understood independently of it. The properties and capabilities exhibited by a complex multicellular organism can be reduced to the properties of the individual cells of that organism, and the behaviour of each individual cell can be understood in terms of its chemical structure and the chemical changes that occur within that cell.

Chemical composition of living matter.

Every living cell contains, in addition to water and salts or minerals, a large number of organic compounds, substances composed of carbon combined with varying amounts of hydrogen and, usually, oxygen as well. Nitrogen, phosphorus, and sulfur are also common constituents. In general, most of the organic matter in a cell can be classified as (1) protein, (2) carbohydrate, and (3) fat or lipid. Nucleic acids and various other organic derivatives are also important constituents. Each class contains a great diversity of individual compounds. There are also many substances that cannot be classified in any of the above categories, although usually not in large quantities.

Proteins are essential to life, not only as structural elements (eg, collagen) and to provide a defence (as antibodies) against invading destructive forces, but also because the essential biocatalysts are proteins. The chemistry of proteins is based on the research of the German chemist Emil Fischer, whose 1882 work showed that proteins are very large molecules, or polymers, made up of about 24 amino acids. Proteins can range in size from small (insulin with a molecular weight of 5,700 (based on the weight of a hydrogen atom as 1)) to very large molecules with molecular weights of over 1,000,000.

The first complete amino acid sequence was determined for the insulin molecule in the 1950s. By 1963 the amino acid chain in the protein enzyme ribonuclease (molecular weight 12,700) had also been determined, with the help of the powerful physical techniques of analysis. of X-ray diffraction. In the 1960s, Nobel Prize winners JC Kendrew and M. F. Perutz, using X-ray studies, built detailed atomic models of the proteins haemoglobin and myoglobin (the respiratory pigment in muscle), which were later confirmed by sophisticated chemical studies. The continuing interest of biochemists in protein structure is based on the fact that the arrangement of chemical groups in space provides important clues about the biological activity of molecules.

Carbohydrates include substances such as sugars, starch, and cellulose. The second quarter of the 20th century saw a startling advance in understanding how living cells handle small molecules, including carbohydrates. Carbohydrate metabolism became elucidated during this period, and the elaborate pathways of carbohydrate breakdown and subsequent storage and utilization were gradually described in terms of cycles (eg, the Embden-Meyerhof glycolytic cycle and the Krebs cycle). ). The involvement of carbohydrates in respiration and muscle contraction was well elaborated in the 1950s. Refinements of the schemes continue.

Fats, or lipids, are a heterogeneous group of organic chemicals that can be extracted from biological material by nonpolar solvents such as ethanol, ether, and benzene. The classic work on the formation of body fat from carbohydrates was done in the early 1850s. Those studies, and subsequent confirmatory evidence, have shown that the conversion of carbohydrates to fat occurs continuously in the body. The liver is the main site of fat metabolism.

The absorption of fat in the intestine was studied as early as the 1930s. It is known that the control of fat absorption depends on a combined action of the secretions of the pancreas and bile salts. Abnormalities of fat metabolism, which give rise to disorders such as obesity and rare clinical conditions, are the subject of much biochemical research. Equally interesting to biochemists is the association between high levels of fat in the blood and the development of arteriosclerosis (“hardening” of the arteries).

Nucleic acids are large, complex compounds of very high molecular weight present in the cells of all organisms and in viruses. They are of great importance in the synthesis of proteins and in the transmission of hereditary information from one generation to the next. Originally discovered as components of cell nuclei (hence their name), it was assumed for many years after their isolation in 1869 that they were found nowhere else. This assumption was not seriously questioned until the 1940s, when it was determined that there are two types of nucleic acid: deoxyribonucleic acid (DNA), in the nuclei of all cells and in some viruses; and ribonucleic acid (RNA), in the cytoplasm of all cells and in most viruses.

The profound biological importance of nucleic acids gradually came to light during the 1940s and 1950s. Attention turned to the mechanism by which protein synthesis and genetic transmission were controlled by nucleic acids (see below, Genes). During the 1960s, experiments were aimed at refining the genetic code. Promising attempts were made in the late 1960s and early 1970s to replicate nucleic acid molecules outside the cell, that is, in the laboratory. By the mid-1980s, genetic engineering techniques had achieved, among other things, in vitro fertilization and DNA recombination (so-called gene splicing).

Evolution and origin of life.

Space exploration beginning in the mid-20th century intensified speculation about the possibility of life on other planets. At the same time, the man was beginning to understand some of the intimate chemical mechanisms used for the transmission of hereditary characteristics. By studying the structure of proteins in different species, it was possible to see how the amino acid sequences of functional proteins (for example, haemoglobin and cytochrome) have been altered during phylogeny (the development of species). It was natural, therefore, for biochemists to regard the problem of the origin of life as a practical one. The synthesis of a living cell from inanimate material was not considered an impossible task for the future.

Transposons Shifting Segments of the Genome

Transposable elements (TEs), which shift segments of the Genome are also known as “jumping genes”. These elements were first identified more than 50 years ago by geneticist Barbara McClintock of the Cold Spring Harbor Laboratory in New York. Biologists were initially sceptical of McClintock’s discovery. However, over the next several decades, it became clear that TEs not only “jump” but are also found in almost all organisms (both prokaryotes and eukaryotes), and usually in large numbers. For example, TEs constitute approximately 50% of the human genome and up to 90% of the maize genome (SanMiguel, 1996).

Retrotransposons

Unlike class 2 elements, class 1 elements, also known as retrotransposons, move through the action of RNA intermediates. In other words, class 1 TEs do not encode transposase; rather, they produce RNA transcripts and then rely on reverse transcriptase enzymes to reverse transcribe the RNA sequences back into DNA, which is then inserted into the target site.

There are two main types of class 1 TEs: LTR retrotransposons, which are characterized by the presence of long terminal repeats (LTRs) at both ends; and non-LTR TE, which lacks repeats. Both the LINE1, or L1, and Alu genes represent non-LTR TE families. L1 elements average about 6 kilobases in length. By contrast, Alu elements average only a few hundred nucleotides, making them a short interspersed transposable element, or SINE.

Alu is particularly prolific, originating in primates and expanding in a relatively short time to about 1 million copies per cell in humans. L1 is also common in humans; although it is not present in as many copies as Alu, its larger size means that this element constitutes approximately 15%-17% of the human genome (Kazazian & Moran, 1998; Slotkin & Martienssen, 2007). In humans, these non-LTR TEs are the only active class of transposons; LTR retrotransposons and DNA transposons are just ancient genomic relics and are not capable of hopping.

Autonomous and non-autonomous transposons

Both class 1 and class 2 TEs can be autonomous or non-autonomous. Autonomous TEs can move on their own, while non-autonomous elements require the presence of other TEs to move. This is because nonautonomous elements lack the transposase or reverse transcriptase gene needed for their transposition, so they must “borrow” these proteins from another element to move. Ac elements, for example, are autonomous because they can move on their own, while Ds elements are not autonomous because they require the presence of Ac to transpose.

What Jumping Genes Do (Besides Jumping)

The fact that about half of the human genome is made up of TEs, with a significant portion of the L1 and Alu retrotransposons, raises an important question: What do all these jumping genes do, besides jump? Much of what a transposon does depends on where it lands. Landing inside a gene can result in a mutation, as was discovered when L1 insertions into the factor VIII gene caused haemophilia (Kazazian et al., 1988). Similarly, a few years later, the researchers found L1 on the APC genes in colon cancer cells, but not on the APC genes in healthy cells in the same individuals. This confirms that L1 is transposed in mammalian somatic cells and that this element could play a causal role in disease development (Miki et al., 1992).

Silencing and Transposons

Unlike L1, most TEs appear to be silent; in other words, these elements do not produce a phenotypic effect nor do they actively move through the genome. At least that has been the general scientific consensus. Some silenced TEs are inactive because they have mutations that affect their ability to move from one chromosomal location to another; others are perfectly intact and capable of movement but are kept inactive by epigenetic defence mechanisms such as DNA methylation, chromatin remodelling, and miRNAs. In chromatin remodelling, for example, chemical modifications to chromatin proteins cause chromatin to shrink so much in certain areas of the genome that genes and TEs in those areas are silenced because transcription enzymes simply can’t access them.

Another example of transposon silencing involves plants of the genus Arabidopsis. Researchers studying these plants have discovered that they contain more than 20 different mutator transposon sequences (a type of transposon identified in maize). In wild-type plants, these sequences are methylated or silenced. However, in plants that are defective for one of the enzymes responsible for methylation, these transposons are transcribed. Furthermore, several different mutant phenotypes have been explored in methylation-deficient plants, and these phenotypes have been linked to transposon insertions (Miura et al., 2001).

Based on studies like these, scientists know that some ETs are epigenetically silenced; in recent years, however, researchers have begun to question whether certain TEs might have a role in epigenetic silencing. Interestingly, it was Barbara McClintock who first speculated that TEs might play this type of regulatory role (McClintock, 1951). Scientists have taken decades to collect enough evidence to consider that perhaps McClintock’s speculation had an ounce of truth.

Transposons can encode siRNAs that mediate their own silencing

Because transposon movement can be destructive, it is not surprising that most transposon sequences in the human genome are silent, allowing this genome to remain relatively stable, despite the prevalence of TE. In fact, the researchers believe that of the 17% of the human genome that is encoded by L1-related sequences, only about 100 active L1 elements remain. Furthermore, the research suggests that even these few remaining active transposons are inhibited from jumping in a variety of ways that go beyond epigenetic silencing.

For example, in human cells, small interfering RNAs (siRNAs), also known as RNAi, can prevent transposition. RNAi is a natural mechanism that eukaryotes often use to regulate gene expression. What is especially interesting about this situation is that the siRNAs that interfere with L1 activity are derived from the 5′ untranslated region (5′ UTR) of LTR L1. Specifically, the 5’UTR of the L1 promoter encodes a sense promoter that transcribes L1 genes, as well as an antisense promoter that transcribes antisense RNA. Yang and Kazazian (2006) showed that this results in homologous sequences that can hybridize, thus forming a double-stranded RNA molecule that can serve as a substrate for RNAi. Furthermore, when the researchers inhibited endogenous siRNA silencing mechanisms, they observed an increase in L1 transcripts, suggesting that L1 transcription is indeed inhibited by siRNA.

Transposons are not always destructive

Not all transposon jumping has harmful effects. Indeed, transposons may drive the evolution of genomes by facilitating translocation of genomic sequences, exon shuffling, and double-strand break repair. Insertions and transpositions can also alter phenotypes and gene regulatory regions. In the case of the medaka fish, for example, the DNA transposon Tol2 is directly related to pigmentation. A highly inbred line of these fish was shown to have a variety of pigmentation patterns.

In the members of this line in which the Tol2 transposon jumped “cleanly” (ie, without removing other parts of the genomic sequence), the fish were albino. But when Tol2 did not jump cleanly from the regulatory region, the result was a wide range of hereditary pigmentation patterns (Koga et al., 2006).

The fact that transposable elements are not always perfectly removed and can take up genomic sequences during the journey has also resulted in a phenomenon scientists call exon shuffling. Exon shuffling results in the juxtaposition of two previously unrelated exons, usually by rearrangement, potentially creating new gene products (Moran et al., 1999).

The ability of transposons to increase genetic diversity, coupled with the ability of the genome to inhibit most TE activity, results in a balance that makes transposable elements an important part of gene evolution and regulation in all organisms that carry these sequences.

Evaporation-Driven Flow in Micropillar Arrays: Transport Dynamics and Chemical Analysis under Varied Sample and Ambient Conditions

Evaporation-Driven Flow in Micropillar Arrays: Transport Dynamics and Chemical Analysis under Varied Sample and Ambient Conditions
Microfluidic circulation in lab-on-a-chip gadgets is usually very delicate to the variable bodily properties of advanced samples, e.g., organic fluids. Here, evaporation-driven fluid transport (transpiration) is achieved in a configuration that’s insensitive to interfacial pressure, salinity, and viscosity over a variety. Micropillar arrays (“pillar cuvettes”) have been preloaded by wicking a identified risky fluid (water) and then including a microliter pattern of salt, surfactant, sugar, or saliva answer to the loading zone.
As the preloaded fluid evaporates, the pattern is reliably drawn from a reservoir by way of the pillar array at a fee outlined by the evaporation of the preloaded fluid (sometimes nL/s). Including a reagent in the preloaded fluid permits photometric reactions to happen on the boundary between the 2 fluids. In this configuration, a photometric sign enhancement is noticed and chemical evaluation is unbiased of each humidity and temperature. The means to reliably transport and sense an analyte in microliter volumes with out concern over salt, surfactant, viscosity (in half), humidity, and temperature is a exceptional benefit for analytical functions.
Polyagglutination is a uncommon entity in immunohematology and unusually presents in a wholesome blood donor. The basic presentation was described in the literature in affiliation with bacterial infections, which end result in the publicity of crypt antigens. Nowadays, polyagglutination is never detected on account of using monoclonal antisera. Our case report describes the presence of Tn polyagglutination in a wholesome grownup blood donor with no prior historical past of any an infection in the current previous.
Immunohematology work-up for incompatible cross-match was performed in the serology lab utilizing commercially procured antisera and column agglutination gel card (Tulip Diagnostics India Pvt. Ltd, Goa, India). The three cell-screening panel was procured commercially (ID Dia cell I, II, III; Bio-Rad, Switzerland), and in-house lectin was ready as per the usual methodology.We have come throughout a case of incompatible cross-match with damaging antibody display screen, auto-control, and Negative direct coombs take a look at. Cross-match with a number of grownup serum and twine serum offers us a clue in the direction of polyagglutination. Further, Polyagglutination was confirmed serologically utilizing anti-A1 lectin and later concludes of Tn kind by lectin ready in-house from Salvia Sclarea.

The prolonged lipid panel assay: a clinically-deployed high-throughput nuclear magnetic resonance methodology for the simultaneous measurement of lipids and Apolipoprotein B

Standard lipid panel assays using chemical/enzymatic strategies measure complete ldl cholesterol (TC), triglycerides (TG), and high-density lipoprotein ldl cholesterol (HDL-C), from that are calculated estimates of low-density lipoprotein ldl cholesterol (LDL-C). These lipid measures are used universally to information administration of atherosclerotic heart problems danger. Apolipoprotein B (apoB) is usually acknowledged to be superior to LDL-C for lipid-lowering therapeutic decision-making, however apoB immunoassays are carried out comparatively sometimes as a result of added analytic value.
The goal of this research was to develop and validate the efficiency of a speedy, high-throughput, reagent-less assay producing an “Extended Lipid Panel” (ELP) that features apoB, utilizing the Vantera® nuclear magnetic resonance (NMR) analyzer platform already deployed clinically for lipoprotein particle and different testing. Partial least squares regression fashions, utilizing as enter an outlined area of proton NMR spectra of plasma or serum, have been created to concurrently quantify TC, TG, HDL-C, and apoB. Large coaching units (n > ~ 1000) of affected person sera analyzed independently for lipids and apoB by chemical strategies have been employed to make sure prediction fashions replicate the extensive lipid compositional variety of the inhabitants. The analytical efficiency of the NMR ELP assay was comprehensively evaluated.
Excellent settlement was demonstrated between chemically-measured and ELP assay values of TC, TG, HDL-C and apoB with correlation coefficients starting from 0.980 to 0.997. Within-run precision research measured utilizing low, medium, and excessive stage serum swimming pools gave coefficients of variation for the four analytes starting from 1.Zero to three.8% for the low, 1.Zero to 1.7% for the medium, and 0.9 to 1.3% for the excessive swimming pools. Corresponding values for within-lab precision over 20 days have been 1.four to three.6%, 1.2 to 2.3%, and 1.Zero to 1.9%, respectively.
Independent testing at three websites over 5 days produced extremely constant assay outcomes. No main interference was noticed from 38 endogenous or exogenous substances examined. Extensive assay efficiency evaluations validate that the NMR ELP assay is environment friendly, strong, and considerably equal to plain chemistry assays for the scientific measurement of lipids and apoB. Routine reporting of apoB alongside commonplace lipid measures may facilitate extra widespread utilization of apoB for scientific decision-making.
Evaporation-Driven Flow in Micropillar Arrays: Transport Dynamics and Chemical Analysis under Varied Sample and Ambient Conditions

Antibody Printing Technologies

Antibody microarrays are routinely employed in the lab and in the clinic for finding out protein expression, protein-protein, and protein-drug interactions. The microarray format reduces the dimensions scale at which organic and biochemical interactions happen, resulting in giant reductions in reagent consumption and dealing with occasions whereas growing general experimental throughput. Specifically, antibody microarrays, as a platform, provide numerous totally different benefits over conventional strategies in the areas of drug discovery and diagnostics. While numerous totally different strategies and approaches have been developed for creating micro and nanoscale antibody arrays, points referring to sensitivity, value, and reproducibility persist.

Advanced Glycation End Product (AGE) Antibody (Biotin)

20-abx271739
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Advanced Glycation End Product (AGE) Monoclonal Antibody

CAU29357-100ul 100ul
EUR 267.8

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EUR 334.3

Advanced glycation end-product

AP79793 1mg
EUR 2640

Advanced glycation end-product

AP80037 1mg
EUR 2640

Advanced glycation end-product

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EUR 2640

Monoclonal Antibody to Advanced Glycation End Product (AGE)

MAB353Ge21 100ul
EUR 279

Polyclonal Antibody to Advanced Glycation End Product (AGE)

PAB353Ge01 100ul
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Advanced Glycation End Product (AGE) Monoclonal Antibody (General)

4-MAB353Ge21
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE)

Advanced Glycation End Product (AGE) Polyclonal Antibody (General)

4-PAB353Ge01
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE)

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), PE

4-MAB353Ge21-PE
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), PE

4-PAB353Ge01-PE
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC

4-MAB353Ge21-APC
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Cy3

4-MAB353Ge21-Cy3
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), FITC

4-MAB353Ge21-FITC
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), HRP

4-MAB353Ge21-HRP
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC

4-PAB353Ge01-APC
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  • 100ul
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Cy3

4-PAB353Ge01-Cy3
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  • 1ml
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), FITC

4-PAB353Ge01-FITC
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), HRP

4-PAB353Ge01-HRP
  • EUR 362.40
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  • 100ul
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

Advanced Glycation End Product (AGE) ELISA

KT-6025 96 tests
EUR 977

Active Advanced Glycation End Product (AGE)

4-APB353Ge01
  • EUR 550.08
  • EUR 271.20
  • EUR 1732.80
  • EUR 657.60
  • EUR 1195.20
  • EUR 444.00
  • EUR 4152.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Advanced Glycation End Product expressed in: Human

Native Advanced Glycation End Product (AGE)

4-NPB353Ge01
  • EUR 442.56
  • EUR 242.40
  • EUR 1329.60
  • EUR 523.20
  • EUR 926.40
  • EUR 372.00
  • EUR 3144.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Advanced Glycation End Product expressed in: Natural extract

Active Advanced Glycation End Product (AGE)

RPU54925-100ug 100ug
EUR 492.8

Active Advanced Glycation End Product (AGE)

RPU54925-1mg 1mg
EUR 2184

Active Advanced Glycation End Product (AGE)

RPU54925-50ug 50ug
EUR 396

Native Advanced Glycation End Product (AGE)

RPU50405-100ug 100ug
EUR 369.6

Native Advanced Glycation End Product (AGE)

RPU50405-1mg 1mg
EUR 1638

Native Advanced Glycation End Product (AGE)

RPU50405-50ug 50ug
EUR 297

Native Advanced Glycation End Product (AGE)

NPB353Ge01 10ug
EUR 120

Advanced Glycation End Product (AGE) Protein

20-abx168580
  • EUR 627.60
  • EUR 292.80
  • EUR 1796.40
  • EUR 727.20
  • EUR 460.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC-Cy7

4-MAB353Ge21-APC-Cy7
  • EUR 675.60
  • EUR 7586.40
  • EUR 2013.60
  • EUR 898.80
  • EUR 378.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC-Cy7

4-PAB353Ge01-APC-Cy7
  • EUR 645.60
  • EUR 7154.40
  • EUR 1905.60
  • EUR 855.60
  • EUR 364.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7.

Advanced Glycation End Product (AGE) CLIA Kit

20-abx490411
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Biotinylated

4-MAB353Ge21-Biotin
  • EUR 370.80
  • EUR 2904.00
  • EUR 860.40
  • EUR 452.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Biotinylated

4-PAB353Ge01-Biotin
  • EUR 358.80
  • EUR 2745.60
  • EUR 820.80
  • EUR 435.60
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

Advanced Glycation End Product (AGE) ELISA Kit

abx255064-96tests 96 tests
EUR 895.2

Advanced Glycation End Product (AGE) ELISA Kit

20-abx150316
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Advanced Glycation End Product (AGE) ELISA Kit

abx576530-96tests 96 tests
EUR 895.2

Advanced Glycation End Product (AGE) ELISA Kit

DLR-AGE-Ge-48T 48T
EUR 627.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Advanced Glycation End Product (AGE) ELISA Kit

DLR-AGE-Ge-96T 96T
EUR 817.2
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Advanced Glycation End Product (AGE) ELISA Kit

EKN52019-48T 48T
EUR 386.26

Advanced Glycation End Product (AGE) ELISA Kit

EKN52019-5x96T 5x96T
EUR 2621.05

Advanced Glycation End Product (AGE) ELISA Kit

EKN52019-96T 96T
EUR 551.8

Advanced Glycation End Product (AGE) ELISA Kit

EKU02193-48T 48T
EUR 564.76

Advanced Glycation End Product (AGE) ELISA Kit

EKU02193-5x96T 5x96T
EUR 3832.3

Advanced Glycation End Product (AGE) ELISA Kit

EKU02193-96T 96T
EUR 806.8

Advanced Glycation End Product (AGE) ELISA Kit

EKU10908-48T 48T
EUR 620.69

Advanced Glycation End Product (AGE) ELISA Kit

EKU10908-5x96T 5x96T
EUR 4211.83

Advanced Glycation End Product (AGE) ELISA Kit

EKU10908-96T 96T
EUR 886.7

AGE(Advanced Glycation End Product) ELISA Kit

ELK1890-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

AGE(Advanced Glycation End Product) ELISA Kit

ELK1890-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Advanced Glycation End Product (AGE) ELISA Kit

EKN43304-48T 48T
EUR 384.93

Advanced Glycation End Product (AGE) ELISA Kit

EKN43304-5x96T 5x96T
EUR 2612.03

Advanced Glycation End Product (AGE) ELISA Kit

EKN43304-96T 96T
EUR 549.9

Advanced Glycation End Products (AGEs) Assay Kit

K929-100 each
EUR 502.8

Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A16262 96T
EUR 700
Description: ELISA

Rat Solube receptor for advanced glycation end products/Endogenous secretory receptor for Advanced Glycation end Products ELisa Kit

E01A16586 96T
EUR 700
Description: ELISA

Bovine Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A86032 96T
EUR 700
Description: ELISA

Rabbit Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A33731 96T
EUR 700
Description: ELISA

Human Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A7517 96T
EUR 700
Description: ELISA

Monkey Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A77312 96T
EUR 700
Description: ELISA

Sheep Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A103459 96T
EUR 700
Description: ELISA

Goat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A51165 96T
EUR 700
Description: ELISA

Canine Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A68593 96T
EUR 700
Description: ELISA

Porcine Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A59883 96T
EUR 700
Description: ELISA

Mouse Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A25009 96T
EUR 700
Description: ELISA

Advanced Glycation End Product (AGE) Protein (Active)

20-abx651294
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Bovine Solube receptor for advanced glycation end products/Endogenous secretory receptor for Advanced Glycation end Products ELisa Kit

E01A86355 96T
EUR 700
Description: ELISA

Human Solube receptor for advanced glycation end products/Endogenous secretory receptor for Advanced Glycation end Products ELisa Kit

E01A7841 96T
EUR 700
Description: ELISA

Mouse Solube receptor for advanced glycation end products/Endogenous secretory receptor for Advanced Glycation end Products ELisa Kit

E01A25332 96T
EUR 700
Description: ELISA

Rat Advanced Glycation End Product (AGE) ELISA Kit

abx256246-96tests 96 tests
EUR 904.8

Rat Advanced Glycation End Product (AGE) ELISA Kit

abx512407-96tests 96 tests
EUR 782.4

for Advanced Glycation End Product (AGE)ELISA kit

HEB353Ge-10x96wellstestplate 10x96-wells test plate
EUR 6321.83
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit

HEB353Ge-1x48wellstestplate 1x48-wells test plate
EUR 625.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit

HEB353Ge-1x96wellstestplate 1x96-wells test plate
EUR 842.57
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit

HEB353Ge-5x96wellstestplate 5x96-wells test plate
EUR 3431.56
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

ELISA Kit for Advanced Glycation End Product (AGE)

4-HEB353Ge
  • EUR 6382.80
  • EUR 3372.00
  • EUR 843.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Cat Advanced Glycation End Product (AGE) ELISA Kit

YLA0032CT-48T 48T
EUR 547.5

Cat Advanced Glycation End Product (AGE) ELISA Kit

YLA0032CT-96T 96T
EUR 637.5

Rat AGE(Advanced Glycation End Product) ELISA Kit

ELK0785-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat AGE(Advanced Glycation End Product) ELISA Kit

ELK0785-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGE in the samples is then determined by comparing the OD of the samples to the standard curve.

ELISA Kit for Advanced Glycation End Product (AGE)

CEB353Ge 96Т
EUR 711

Guinea Pig Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit

E01A42448 96T
EUR 700
Description: ELISA

Human Advanced Glycation End Product (AGE) ELISA Kit

abx253588-96tests 96 tests
EUR 801.6

Human Advanced Glycation End Product (AGE) ELISA Kit

20-abx054077
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Advanced Glycation End Product (AGE) ELISA Kit

abx512405-96tests 96 tests
EUR 801.6

Mouse Advanced Glycation End Product (AGE) ELISA Kit

abx512406-96tests 96 tests
EUR 782.4

Rat advanced glycation end products,AGEs ELISA Kit

CN-01677R1 96T
EUR 549.6

Rat advanced glycation end products,AGEs ELISA Kit

CN-01677R2 48T
EUR 368.4

Rat advanced glycation end products, AGEs ELISA Kit

CSB-E09413r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat advanced glycation end products, AGEs ELISA Kit

1-CSB-E09413r
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat AGE(Advanced glycation end-product) ELISA Kit

ER0268 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Rat AGE/ Advanced glycation end-product ELISA Kit

E0037Ra 1 Kit
EUR 685.2

Rat advanced glycation end products(AGEs)ELISA Kit

GA-E0614RT-48T 48T
EUR 380.4

Rat advanced glycation end products(AGEs)ELISA Kit

GA-E0614RT-96T 96T
EUR 595.2

Rat advanced glycation end products, AGEs ELISA Kit

ELA-E0263r 96 Tests
EUR 1063.2

Rat advanced glycation end products,AGEs ELISA Kit

SL0036Ra -
EUR 498

Rat Advanced glycation end-product (AGE) ELISA Kit

RK06498 96T
EUR 280

Rat AGE(Advanced glycation end-product) ELISA Kit

EKF57894-48T 48T
EUR 396.9

Rat AGE(Advanced glycation end-product) ELISA Kit

EKF57894-5x96T 5x96T
EUR 2693.25

Rat AGE(Advanced glycation end-product) ELISA Kit

EKF57894-96T 96T
EUR 567

Mouse AGE(advanced glycation end product) ELISA Kit

EKF58424-48T 48T
EUR 396.9

Mouse AGE(advanced glycation end product) ELISA Kit

EKF58424-5x96T 5x96T
EUR 2693.25

Mouse AGE(advanced glycation end product) ELISA Kit

EKF58424-96T 96T
EUR 567

Rat advanced glycation end products,AGEs ELISA Kit

YLA0170RA-48T 48T
EUR 465

Rat advanced glycation end products,AGEs ELISA Kit

YLA0170RA-96T 96T
EUR 600

Rat Advanced glycation end products (AGEs) ELISA Kit

AE24247RA-48Tests 48 Tests
EUR 360
Description: Rat (Rattus norvegicus)

Rat Advanced glycation end products (AGEs) ELISA Kit

AE24247RA-96Tests 96 Tests
EUR 680
Description: Rat (Rattus norvegicus)

Rat Advanced glycation end products(AGEs)ELISA Kit

NSL1681r 96 Tests
EUR 498

Rat advanced glycation end products(AGEs)ELISA Kit

QY-E11038 96T
EUR 433.2

Rat advanced glycation end products(AGEs) Elisa Kit

EK720191 96 Wells
EUR 0.54

Human advanced Glycation end product (AGE) Elisa kit

YLA4301HU-48T 48T
EUR 435

Human advanced Glycation end product (AGE) Elisa kit

YLA4301HU-96T 96T
EUR 562.5

Rat advanced glycation end products,AGEs ELISA Kit

EKC38623-48T 48T
EUR 647.15

Rat advanced glycation end products,AGEs ELISA Kit

EKC38623-5x96T 5x96T
EUR 4391.38

Rat advanced glycation end products,AGEs ELISA Kit

EKC38623-96T 96T
EUR 924.5

Rat Advanced glycation end products (AGEs) ELISA Kit

EK7782 96Т
EUR 799

Goat Advanced glycation end products(AGEs) ELISA Kit

NSL1405Gt 96T
EUR 528

Human advanced glycation end products,AGEs ELISA Kit

201-12-0004 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit

CEB353Ge-10x96wellstestplate 10x96-wells test plate
EUR 5764.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit

CEB353Ge-1x48wellstestplate 1x48-wells test plate
EUR 579.88
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit

CEB353Ge-1x96wellstestplate 1x96-wells test plate
EUR 776.96
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit

CEB353Ge-5x96wellstestplate 5x96-wells test plate
EUR 3136.34
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit

4-CEB353Ge
  • EUR 5824.80
  • EUR 3076.80
  • EUR 777.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Advanced Glycation End Product (AGE) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse advanced glycation end products,AGEs ELISA Kit

CN-02549M1 96T
EUR 565.2

Mouse advanced glycation end products,AGEs ELISA Kit

CN-02549M2 48T
EUR 386.4

Human advanced glycation end products,AGEs ELISA Kit

CN-04128H1 96T
EUR 567.6

Human advanced glycation end products,AGEs ELISA Kit

CN-04128H2 48T
EUR 387.6

Human advanced glycation end products, AGEs ELISA Kit

CSB-E09412h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human advanced glycation end products, AGEs ELISA Kit

1-CSB-E09412h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse advanced glycation end products, AGEs ELISA Kit

CSB-E09414m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse advanced glycation end products, AGEs ELISA Kit

1-CSB-E09414m
  • EUR 1135.20
  • EUR 6938.40
  • EUR 3672.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse advanced glycation end products, AGEs ELISA Kit

E0263m 96 Tests
EUR 813.6

Mouse AGE/ Advanced glycation end-product ELISA Kit

E0051Mo 1 Kit
EUR 685.2

Human AGE/ Advanced glycation end-product ELISA Kit

E0080Hu 1 Kit
EUR 685.2

Mouse advanced glycation end products(AGEs)ELISA Kit

GA-E0602MS-48T 48T
EUR 403.2

Mouse advanced glycation end products(AGEs)ELISA Kit

GA-E0602MS-96T 96T
EUR 640.8

Human advanced glycation end products(AGEs)ELISA Kit

GA-E0051HM-48T 48T
EUR 346.8

Human advanced glycation end products(AGEs)ELISA Kit

GA-E0051HM-96T 96T
EUR 559.2

Human AGE(Advanced glycation end-product) ELISA Kit

EH0622 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

ELISA kit for Mouse Advanced glycation end-product

EK5086 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Advanced glycation end-product in samples from serum, plasma, tissue homogenates and other biological fluids.

Human Advanced Glycation End products, AGEs ELISA Kit

SL0077Hu 96 Tests
EUR 468

The goal of this assessment is to spotlight present state-of the-art strategies and approaches for creating antibody arrays by offering newest accounts of the sphere whereas discussing potential future instructions. Our outcomes point out that gram quantities of anti-SARS-CoV-2 antibodies could possibly be simply produced in little greater than 6 weeks in repurposed greenhouses with little infrastructure necessities utilizing N. benthamiana as manufacturing platform. Similar procedures could possibly be simply deployed to provide diagnostic reagents and, ultimately, could possibly be tailored for fast therapeutic responses.

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

The present CoVid-19 disaster is revealing the strengths and the weaknesses of the world’s capability to answer a world well being disaster. A important weak spot has resulted from the extreme centralization of the present biomanufacturing capacities, a matter of nice concern, if not a supply of nationalistic tensions. On the optimistic facet, scientific knowledge and data have been shared at an unprecedented pace fuelled by the preprint phenomena, and this has significantly strengthened our capability to develop new technology-based options.

In this work, we discover how, in a context of fast change of scientific info, plant biofactories can function a fast and simply adaptable answer for native manufacturing of bioreagents, extra particularly recombinant antibodies. For this objective, we examined our capability to provide, in the framework of an educational lab and in a matter of weeks, milligram quantities of six totally different recombinant monoclonal antibodies in opposition to SARS-CoV-2 in Nicotiana benthamiana.

For the design of the antibodies, we took benefit, amongst different knowledge sources, of the DNA sequence info made quickly available by different teams in preprint publications. mAbs have been engineered as single-chain fragments fused to a human gamma Fc and transiently expressed utilizing a viral vector. In parallel, we additionally produced the recombinant SARS-CoV-2 N protein and the receptor binding area (RBD) of the Spike protein in planta and used them to check the binding specificity of the recombinant mAbs. Finally, for two of the antibodies, we assayed a easy scale-up manufacturing protocol based mostly on the extraction of apoplastic fluid.

An environmentally-benign flow-batch system for headspace single-drop microextraction and on-drop conductometric detecting ammonium

This work presents a lab-made automated flow-batch system for headspace single-drop microextraction and on-drop conductometric sensing ammonium. Sample and NaOH answer are concurrently pumped right into a response chamber (RC), the place ammonium is transformed to ammonia by elevating pH. The transformed ammonia then diffuses into the headspace of the RC, and reacts with a 100 mM boric acid drop. The conductivity of the drop is measured by an on-drop conductivity probe, which is made by two stainless-steel contacting electrodes.

The end result exhibits that the growing charge of conductivity has a linear relationship to the ammonium focus in pattern (R2 = 0.9945). This methodology has a linear vary as much as 400 μM, a restrict of detection 2.eight μM, a relative normal deviation of 3.0% (200 μM, n = 10) and carryover coefficient 0.028. Measurements of river waters, lake waters and wastewaters have been demonstrated. The recoveries have achieved from 99.Zero to 114%. This methodology avoids utilizing of dangerous or odorous reagents and follows the idea of inexperienced chemistry.

The worldwide COVID-19 pandemic outburst has triggered a severe public well being problem with growing wants of correct and fast diagnostic and screening testing. This scenario requires an optimized administration of the chemical reagents, the consumables, and the human assets, in order to reply precisely and successfully, controlling the unfold of the illness. Testing on pooled samples maximizes the quantity of examined samples, by minimizing the time and the lab provides wanted. The normal conceptualization of the pooling methodology is predicated on mixing samples collectively in a batch.

Individual testing is required provided that a particular pool displays a optimistic end result. The growth of various hybrid strategies, based mostly on “in home” protocols, using commercially available consumables, in mixture with a dependable pooling methodology would supply an answer, specializing in the higher exploitation of the personnel and the lab provides, permitting for fast screening of a inhabitants in a fairly brief time.

Pilot Production of SARS-CoV-2 Related Proteins in Plants: A Proof of Concept for Rapid Repurposing of Indoor Farms Into Biomanufacturing Facilities

Development of novel lab-on-a-chip platform for high-throughput radioimmunoassay

Radioimmunoassay (RIA) is an especially particular and a extremely delicate sort of immunoassay, however the lengthy incubation time and technology of radioactive wastes restrict the use of RIA. To complement these disadvantages of RIA, we advise a sophisticated sort of RIA based mostly on a lab-on-a-chip (LOC) platform: μ-RIA. We designed a microfluidic chip for RIA and optimized the procedures of μ-RIA evaluation, together with floor modification, immunoreaction time, and washing. Based on the optimized situations, we performed a radioimmunoassay on the μ-RIA platform utilizing a business RIA package.

With the μ-RIA, 5 min are sufficient for evaluation. The quantity of reagent consumption is considerably diminished in contrast with standard RIA. The normal curve with R2 = 0.9951 exhibits that we are able to quantitatively consider the quantity of antigen current in unknown samples. We present the applicability of μ-RIA for the evaluation of biomolecules and the potential of μ-RIA to be a novel platform for high-throughput evaluation.

OOSA10426-20ML - SERUM:Azide Free Serum & Cells

OOSA10426-20ML 20ml
EUR 99

OOSA10430-10ML - SERUM:Azide Free Serum & Cells

OOSA10430-10ML 10ml
EUR 379

OOSA10419-100ML - SERUM:Azide Free Serum & Cells

OOSA10419-100ML 100ml
EUR 749

OOSA11794-100ML - SERUM:Azide free Serum & Cells

OOSA11794-100ML 100ml
EUR 1489

OOSA10413-5ML - Serum:Azide Free

OOSA10413-5ML 5ml
EUR 69

OOSA10415-10ML - SERUM:Azide Free

OOSA10415-10ML 10ml
EUR 139

OOSA10428-100ML - SERUM:Azide Free

OOSA10428-100ML 100ml
EUR 139

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

Free Chlorine Reagents 0-5mg/L

WAT1028 EACH
EUR 44.4

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

EZ-DNA Reagents

BS8202 100preps
EUR 105.67

EZ-DNA Reagents

SK8201 100preps
EUR 112.2

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0

7917-5 each
EUR 144

Albumin Depletion Reagent for Plasma and Serum (20 ml)

P5W13 - Ask for price

Ecl Detection Reagents

RPN2105 EACH
EUR 724.8

Ecl Detection Reagents

RPN3004 EACH
EUR 582

OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade

OPEF01351-100G 100g
EUR 1302

MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml)

WA-013 each
EUR 135

REAGENTS HIGH RANGE AMMONIA

HI73325 PK25
EUR 49.2

High Range Ammonia Reagents

HI9373301 EACH
EUR 116.4

Human IgG (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-1 1 mg
EUR 196.8

CryoProtX Mix Reagents 1.5 mL

M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Alkphos Direct Labelling Reagents

RPN3680 EACH
EUR 813.6

Set of 10 Biolipidure Reagents

Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Steroid Free Serum

90R-1007 50 mL
EUR 470.4
Description: Steroid Free Human Serum

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)

20007-1-LE-BTN 100 ug
EUR 343.2

CryoProtX Mix Eco Reagents 1.5 mL

M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

PeliKine tool set, additional reagents

M1980 1 unit
EUR 177.88

OOSA10429-10ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10429-10ML 10ml
EUR 279

OOSA10433-100ML - Rat SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10433-100ML 100ml
EUR 1799

Human Serum (CRP free)

90R-100 100 ml
EUR 1326
Description: C-reactive protein free normal human serum

Human Serum (FABP free)

90R-109 100 ml
EUR 1326
Description: FABP free normal human serum

Bradford(Coomassie) Protein Assay Plus Reagents

P7201-050 450ml
EUR 225.6

HEK 293 Media, Serum Free

TBS8084-1L 1L
EUR 98

Human Serum (Myoglobin Free)

90R-110 100 ml
EUR 1326
Description: Myoglobin free normal human serum

OOMA00062-50ML - CRP Free Serum

OOMA00062-50ML 50ML
EUR 1379

Serum Free Medium For Rat Neural Stem Cells

RAXNF-90011 100mL
EUR 289

Human Serum (Troponin I Free)

90R-106 100 ml
EUR 1417.2
Description: Troponion I free normal human serum

Serum Free Cryopreservation Medium

TM026 25 ml
EUR 50

500mL INSECTAGRO SF9 serum free

13-410-CV PK6
EUR 222

Bovine Serum Albumin, RNase Free

40200064-1 1 mL
EUR 33.37
Description: BSA

Serum Free Complete Medium For Rat Fetal Neuron

RAXFN-90011 100mL
EUR 250

Serum Albumin, Lipid Free Protein

20-abx260049
  • EUR 927.60
  • EUR 343.20
  • EUR 627.60
  • 100 mg
  • 10 mg
  • 50 mg

Bovine Serum Albumin, protease free

GK4012-1KG 1 kg
EUR 1165.2

Bovine Serum Albumin, protease free

GK4012-500G 500 g
EUR 638.4

Albumin, Bovine Serum, Globulin Free

01281-26 100G
EUR 265.3

Albumin, Bovine Serum, Globulin Free

01281-84 50G
EUR 153.3

Albumin, Bovine Serum, Globulin Free

01281-97 10G
EUR 43.4

Bovine Serum Albumin, protease free

GK4012-1 1
EUR 2065.2

Bovine Serum Albumin, protease free

GK4012-500 500
EUR 1181.9

OOSA10412-5ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10412-5ML 5ml
EUR 69

OOSA10414-10ML - SERUM WITH 0.09% AZIDE Serum & Cells

OOSA10414-10ML 10ml
EUR 149

Purified rat serum albumin protein (RSA, >99%, globulin free)

ALBR13-N-10 10 mg
EUR 343.2

Bovine Serum Albumin, fatty acid free

GX5685-1KG 1 kg
EUR 1442.4

Bovine Serum Albumin, fatty acid free

GX5685-500G 500 g
EUR 792

Bovine Serum Albumin, fatty acid free

GX5685-1 1
EUR 1162.8

Bovine Serum Albumin, fatty acid free

GX5685-500 500
EUR 624.9

Freezing Medium (Serum-free & animal origin-free)

PB180438-100mL 100 mL
EUR 118
Description: Supplements & Reagents

Freezing Medium (Serum-free & animal origin-free)

PB180438-50mL 50 mL
EUR 65
Description: Supplements & Reagents

Normal Bovine Serum (Protease/IgG free)

88R-1012 10 ml
EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Albumin, Bovine Serum, Protease Free, pH5.2

01862-74 10G
EUR 53.9

Albumin, Bovine Serum, Protease Free, pH5.2

01862-87 100G
EUR 178.5

Human Apolipoprotein AI / B Free Serum Protein

abx060869-100ml 100 ml
EUR 594

Amersham ECL Western Blotting Detection Reagents for 4000cm2 membrane

RPN2106 EACH
EUR 374.4

Amersham ECL Western Blotting Detection Reagents for 1000cm2 membrane

RPN2109 EACH
EUR 188.4

Amersham ECL Western Blotting Detection Reagents for 6000cm2 membrane

RPN2134 1KIT
EUR 572.4

Amersham ECL Western Blotting Detection Reagents for 2000cm2 membrane

RPN2209 EACH
EUR 291.6

OPMA04391-50ML - Troponin I Free Serum Protein

OPMA04391-50ML 50ML
EUR 1299

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-26 10G
EUR 63.7

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-42 25G
EUR 137.9

Albumin, Bovine Serum, Fatty Acid Free, pH7.0

08587-84 50G
EUR 184.1

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 5nm

22716-100 100ml
EUR 932
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 10nm

22717-100 100ml
EUR 919
Description: 7732-18-5

ImmunoGold labeling reagents for (TEM) - Unconjugated gold colloid (GC), 15nm

22718-100 100ml
EUR 920
Description: 7732-18-5

ImmunoGold labeling reagents for (SEM) - Unconjugated gold colloid (GC) 20nm

22719-100 100ml
EUR 932

Corning Lymphocyte Serum Free Medium KBM551

88-551-CM 1L
EUR 201.6

Corning Lymphocyte Serum Free Medium KBM581

88-581-CM 1L
EUR 266.4

Fetal Bovine Serum, Premium Tetracyclin Free

F0500-050 500ml
EUR 1388.4

Fetal Bovine Serum (EU Origin). Tetracycline free - 100ml

FB-1280T/100 100ml
EUR 56.82

Fetal Bovine Serum (EU Origin). Tetracycline free - 500ml

FB-1280T/500 500ml
EUR 220

Serum Free Medium For Mouse Neural Stem Cells

MUXNF-90011 100mL
EUR 289

Bovine Serum Albumin Protease Free Lyophilised - kg

PM-T1726/1000 kg
EUR 549.29

Fetal Bovine Serum (South America). Tetracycline free - 100ml

FB-1001T/100 100ml
EUR 56.82

Fetal Bovine Serum (South America). Tetracycline free - 500ml

FB-1001T/500 500ml
EUR 220

Bovine Serum Albumin Protease Free Lyophilised - 100g

PM-T1726/100 100g
EUR 111.21

Bovine Serum Albumin Protease Free Lyophilised - 500g

PM-T1726/500 500g
EUR 339.79

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-1 5 g
EUR 22.36
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-2 10 g
EUR 27.79
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-3 25 g
EUR 51.82
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-4 50 g
EUR 78.47
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-5 100 g
EUR 116.35
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-6 250 g
EUR 206.54
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-7 500 g
EUR 358.96
Description: BSA

Bovine Serum Albumin, Fraction V - Fatty Acid Free

22070023-8 1 kg
EUR 583.54
Description: BSA

Goat Serum (10%) in PBST with Azide

30611260-1 100 ml
EUR 33.41

Goat Serum (10%) in PBST with Azide

30611260-2 250 ml
EUR 59.36

Purified rat serum albumin protein (RSA, >99%, fatty acid and globulin free)

ALBR14-N-10 10 mg
EUR 416.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 724

Horse Serum (10%) in PBST with Azide

30611278-1 100 ml
EUR 31.76

Horse Serum (10%) in PBST with Azide

30611278-2 250 ml
EUR 59.79

Fetal Bovine Serum (EU Origin). Tetracycline free - 50ml

FB-1280T/50 50ml
EUR 45.49

Bovine Serum Albumin Fatty Acids Free Lyophilised - kg

PM-T1727/1000 kg
EUR 1136.69

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Protease Free, pH 7.0

7919-5 each
EUR 144

Serum Free Medium For Human Fetal Neural Stem Cells

HUXNF-90011 100mL
EUR 289

Bovine Serum Albumin Fatty Acids Free Lyophilised - 100g

PM-T1727/100 100g
EUR 155.21

Bovine Serum Albumin Fatty Acids Free Lyophilised - 500g

PM-T1727/500 500g
EUR 574.42

HSA Human Serum Albumin Recombinant Protein, Lipid Free

PROTP02768-3 Regular: 50mg
EUR 565.2
Description: HSA Human Recombinant lipid reduced produced in Plant is a non-glycosylated, polypeptide chain containing 585 amino acids and having a molecular mass of 67 kDa.

SuperKine™ Serum/Protein-Free Cell Freezing Medium

BMU108-EN-100mL 100 mL
EUR 109
Description: SuperKine™ Serum/Protein-Free Cell Freezing Medium is a kind of non programmed, ready-to-use cell freezing medium with clear formula, serum-free and protein-free, which is applicable to most mammalian cells. It can eliminate the potential pathogen contamination or immune reaction risk of serum or protein, so it is safer to use, no need for programmed cooling, and can keep the recovery survival rate of various cells above 95%.

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-1 100 ml
EUR 31.76

Horse Serum (10%) in PBS with Azide, pH 7.4

40120170-2 250 ml
EUR 54.54

Fetal Bovine Serum (South America). Tetracycline free - 50ml

FB-1001T/50 50ml
EUR 45.49

Free Chlorine Reagent set (25 tests)

HI-701-25 PK25
EUR 10.8

Xpro IP Cell Lysis Reagent,SDS Free

X3100-010 100ml
EUR 208.8

Xpro IP Cell Lysis Reagent,SDS Free

X3100-050 500ml
EUR 380.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-100 each
EUR 1358.4

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-25 each
EUR 535.2

Bovine Serum Albumin ? Heat Shock, Fatty Acid Free, pH 7.0

7921-5 each
EUR 210

cAMP Biotrak(TM) EIA; (Non-Acetylation Protocol; Lysis Reagents not Included); Cytiva; RPN2251

GERPN2251 EACH
EUR 685.2

30(w/v)%-Albumin, Bovine Serum, Solution, Fatty Acid Free

19361-84 20ML
EUR 105

Goat Serum (10%) in TBST Buffer with Azide

30611263-1 100 mL
EUR 33.41

Goat Serum (10%) in TBST Buffer with Azide

30611263-2 250 ml
EUR 57.19

EP Reagent Carbon Dioxide Free Water

1095502 1L
EUR 76.8

EP Reagent Carbon Dioxide Free Water

1095502TO 1L
EUR 124.03

Serum Free Medium (Type II) For Mouse Embryonic Stem Cells

MUXES-90061 200mL
EUR 839

Serum Free Medium (Type I) For Mouse Embryonic Stem Cells

MUXES-90062 200mL
EUR 839

Human Serum Albumin Recombinant Protein Tag Free Lyophilized

IHUSERALBRTFLY50UG each
EUR 398
Description: Human Serum Albumin Recombinant Protein Tag Free Lyophilized

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-100 each
EUR 1096.8

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-1000 each Ask for price

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-25 each
EUR 444

Bovine Serum Albumin ? Heat Shock, Protease DNASE Free, pH 7.0

7920-5 each
EUR 183.6

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Tissue Culture Media

EXOFLOW2-BASICA-TC 30 rxn
EUR 1201.2

Serum-Free 293 Media

TM099 500ml
EUR 50

Human Lambda Light Chain (free) goat polyclonal antibody, Serum

AP31533SU-N 1 ml Ask for price

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-1 100 ml
EUR 33.41

Goat Serum (10%) in TBST Wash Buffer with Azide

30611266-2 250 ml
EUR 57.11

Serum-Free CHO Media

TM098 500ml
EUR 50

Rat Serum

abx098267-10ml 10 ml
EUR 326.4

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-1 1 ml
EUR 343.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-10 10 ml
EUR 781.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)

NSPF-5 5 ml
EUR 634.8

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-100 each
EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-25 each
EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 7.0

7907-5 each
EUR 144

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-100 each
EUR 783.6

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-25 each
EUR 294

Bovine Serum Albumin ? Cohn Fraction V, Fatty Acid Free, pH ? 5.2

7908-5 each
EUR 144

HaCaT Cells (Serum-Free)

T0020001.1 One Frozen vial
EUR 570

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-1 100 ml
EUR 32.94

Sheep Serum (10%) in PBST with Azide, pH 7.4, Sterile

30611251-2 250 ml
EUR 59.44

Donkey Serum (10%) in PBST and Azide, pH 7.4, Sterile

30611269-1 50 mL
EUR 36.77

New platforms are enabling radiochemistry to be carried out in tiny, microliter-scale volumes, and this functionality has monumental advantages for the manufacturing of radiopharmaceuticals. These droplet-based applied sciences can obtain comparable or higher yields in comparison with standard strategies, however with vastly diminished reagent consumption, shorter synthesis time, larger molar exercise (even for low exercise batches), quicker purification, and ultra-compact system dimension. We evaluate right here the state of the artwork of this rising route, summarize the radiotracers and prosthetic teams which were synthesized in droplet format, describe latest achievements in scaling up exercise ranges, and talk about benefits and limitations and the long run outlook of these modern gadgets.

CRISPR Systems for COVID-19 Diagnosis

CRISPR Systems for COVID-19 Diagnosis

The emergence of the brand new coronavirus 2019 (COVID-19) was first seen in December 2019, which has unfold quickly and turn into a world pandemic. The variety of instances of COVID-19 and its related mortality have raised severe issues worldwide. Early analysis of viral an infection undoubtedly permits fast intervention, illness administration, and substantial management of the fast unfold of the illness. The methodology offered right here is helpful for routine growth of tissue slices and adherent or floating cultured cells, and in addition types the idea for these variant strategies.

Currently, the usual method for COVID-19 analysis globally is the RT-qPCR take a look at; nevertheless, the restricted entry to kits and related reagents, the necessity for specialised lab tools, and the necessity for extremely expert personnel has led to a detection slowdown. Recently, the event of clustered often interspaced brief palindromic repeats (CRISPR)-based diagnostic methods has reshaped molecular analysis. The advantages of the CRISPR system resembling velocity, precision, specificity, energy, effectivity, and flexibility have impressed researchers to develop CRISPR-based diagnostic and therapeutic strategies.

With the worldwide COVID-19 outbreak, totally different teams have begun to design and develop diagnostic and therapeutic applications based mostly on the environment friendly CRISPR system. CRISPR-based COVID-19 diagnostic methods have benefits resembling a excessive detection velocity (i.e., 30 min from uncooked pattern to achieve a consequence), excessive sensitivity and precision, portability, and no want for specialised laboratory tools. Here, we assessment up to date research on the detection of COVID-19 based mostly on the CRISPR system.

A Streamlined Whole Blood CyTOF Workflow Defines A Circulating Immune Cell Signature of COVID-19

Mass cytometry (CyTOF) represents some of the highly effective instruments in immune phenotyping, permitting excessive throughput quantification of over 40 parameters at single-cell decision. However, extensive deployment of CyTOF-based immune phenotyping research are restricted by complicated experimental workflows and the necessity for specialised CyTOF tools and technical experience. Furthermore, variations in cell isolation and enrichment protocols, antibody reagent preparation, pattern staining, and information acquisition protocols can all introduce technical variation that may confound integrative analyses of enormous data-sets of samples processed throughout a number of labs.

Here, we current a streamlined entire blood CyTOF workflow which addresses many of those sources of experimental variation and facilitates wider adoption of CyTOF immune monitoring throughout websites with restricted technical experience or sample-processing sources or tools. Our workflow makes use of commercially obtainable reagents together with the Fluidigm MaxPar Direct Immune Profiling Assay (MDIPA), a dry tube 30-marker immunophenotyping panel, and SmartTube Proteomic Stabilizer, which permits for easy and dependable fixation and cryopreservation of entire blood samples.

We validate a workflow that permits for streamlined staining of entire blood samples with minimal processing necessities or experience on the web site of pattern assortment, adopted by cargo to a central CyTOF core facility for batched downstream processing and information acquisition. We apply this workflow to characterize 184 entire blood samples collected longitudinally from a cohort of 72 hospitalized COVID-19 sufferers and wholesome controls, highlighting dynamic disease-associated adjustments in circulating immune cell frequency and phenotype.

Super-resolution microscopy strategies circumvent the classical diffraction restrict of optical microscopy utilizing combos of specifically engineered excitation mild, fluorescent dyes, extremely delicate detectors, and reconstruction algorithms. Protein-retention growth microscopy (ExM) is a technique to bodily broaden organic specimens, enabling successfully sub-diffraction restricted imaging on normal microscopes with normal staining reagents. Specimen growth is pushed by a swellable gel materials that may be synthesized in situ utilizing off-the-shelf chemical substances and supplies. The growth materials and course of are strong and amenable to additional growth, which has enabled the emergence of quite a few ExM variants with prolonged capabilities from a number of unbiased labs.

CRISPR Systems for COVID-19 Diagnosis

Predicting the COVID-19 an infection with fourteen medical options utilizing machine studying classification algorithms

While the RT-PCR is the silver bullet take a look at for confirming the COVID-19 an infection, it’s restricted by the dearth of reagents, time-consuming, and the necessity for specialised labs. As another, many of the prior research have centered on Chest CT photographs and Chest X-Ray photographs utilizing deep studying algorithms. However, these two approaches can not at all times be used for sufferers’ screening because of the radiation doses, excessive prices, and the low variety of obtainable gadgets. Hence, there’s a want for a cheaper and sooner diagnostic mannequin to determine the constructive and detrimental instances of COVID-19.

OOSA10413-5ML - Serum:Azide Free

OOSA10413-5ML 5ml
EUR 69

OOSA10415-10ML - SERUM:Azide Free

OOSA10415-10ML 10ml
EUR 139

OOSA10428-100ML - SERUM:Azide Free

OOSA10428-100ML 100ml
EUR 139

Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma

EXOFLOW2-BASICA-SP 30 rxn
EUR 1201.2

Guinea Pig Serum - 100ml

GU-310/100 100ml
EUR 194.92

Guinea Pig Serum - 500ml

GU-310/500 500ml
EUR 721.11

Free Chlorine Reagents 0-5mg/L

WAT1028 EACH
EUR 44.4

Guinea Pig Serum - 50ml

GU-310/50 50ml
EUR 97.79

Normal Guinea Pig Serum

88-NP25 50 ml
EUR 264
Description: Normal Guinea Pig Serum

Normal Guinea Pig Serum

88R-1016 5 ml
EUR 170.4
Description: Normal Guinea Pig Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Guinea Pig Serum Albumin

30R-3306 10 mg
EUR 392.4
Description: Purified native Guinea Pig serum albumin

Guinea pig serum albumin

CSB-NP002001Gp 10mg Ask for price

Guinea Pig Complement Serum

32R-CP004 5 ml
EUR 457.2
Description: Purified Guinea Pig Complement adsorbed with mouse cells

Guinea Pig Complement Serum

32R-CP006 3 ml
EUR 211.2
Description: Purified Guinea Pig Complement isolated from the blood of normal Hartley guinea pigs

Guinea Pig Complement Serum

32R-CP007 25 ml
EUR 807.6
Description: Purified Guinea Pig Complement adsorbed with sheep red blood cells

Guinea Pig Serum Proteins goat polyclonal antibody, Serum

AP31481SU-N 1 ml Ask for price

Guinea Pig Serum Proteins sheep polyclonal antibody, Serum

AP31463SU-N 1 ml Ask for price

Non-sterile guinea pig serum

GPS05-0050 50 ml
EUR 277.68

Non-sterile guinea pig serum

GPS05-0100 100 ml
EUR 332.28

Non-sterile guinea pig serum

GPS05-0500 500 ml
EUR 667.68

Guinea Pig Serum Proteins rabbit polyclonal antibody, Serum

AP31468SU-N 1 ml Ask for price

OORA00623-5ML - GUINEA PIG SERUM

OORA00623-5ML 5mL
EUR 269

Guinea Pig Serum Albumin antibody

70R-15101 100 ug
EUR 392.4
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody

OORA00649-50ML - GUINEA PIG SERUM

OORA00649-50ML 50mL
EUR 869

OORA01197-50ML - GUINEA PIG SERUM

OORA01197-50ML 50mL
EUR 969

OORA00650-100ML - GUINEA PIG SERUM

OORA00650-100ML 100mL
EUR 1349

OORA01281-100ML - GUINEA PIG SERUM

OORA01281-100ML 100mL
EUR 1429

Guinea pig Serum Iron ELISA kit

E05S0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Iron ELISA kit

E05S0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Iron ELISA kit

E05S0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Albumin, Lyophilized

22070080-1 50 mg
EUR 168.11

Guinea Pig Serum Albumin, Lyophilized

22070080-2 100 mg
EUR 272.69

Guinea Pig Serum Albumin, Lyophilized

22070080-3 500 mg
EUR 728.41

Guinea Pig Serum Albumin, Lyophilized

22070080-4 1000 mg
EUR 1147.51

Guinea pig Serum Albumin ELISA kit

E05S0014-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit

E05S0014-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Albumin ELISA kit

E05S0014-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid ELISA kit

E05S0228-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Albumin ELISA kit

E01A42617 96T
EUR 700
Description: ELISA

Guinea Pig Serum Albumin antibody (HRP)

60R-1144 100 ug
EUR 392.4
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (HRP)

Guinea Pig Serum Albumin antibody (FITC)

60R-1145 100 ug
EUR 392.4
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (FITC)

Guinea Pig Serum Albumin antibody (biotin)

60R-1146 100 ug
EUR 392.4
Description: Rabbit polyclonal Guinea Pig Serum Albumin antibody (biotin)

Guinea Pig Serum amyloid A ELISA Kit

ELA-E0885Gu 96 Tests
EUR 1113.6

Guinea Pig Serum amyloid A ELISA kit

E01A42729 96T
EUR 700
Description: ELISA

Guinea pig Serum Albumin(BSA) ELISA Kit

NSL1189Gp 96T
EUR 528

Guinea pig serum albumin lyophilized powder

GPSA62-0050 50mg
EUR 460.2

Guinea pig serum albumin lyophilized powder

GPSA62-0100 100mg
EUR 611.52

Guinea pig serum albumin lyophilized powder

GPSA62-0500 500mg
EUR 1361.88

Guinea pig serum albumin lyophilized powder

GPSA62-1000 1gm
EUR 2388.36

Guinea Pig Serum Albumin, 10mg/mL, Sterile

31114007-1 1 mL
EUR 87.58

Guinea Pig Serum Albumin Polyclonal Antibody

A57556
  • EUR 684.66
  • EUR 117.70
  • EUR 302.50
  • EUR 423.50
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

Guinea Pig control serum (non-immunized) Mixed breed, Mouse serum -ve & G. Pig +ve

NGPS-25-GM 25 ml
EUR 489.6

VIP guinea pig polyclonal antibody, Serum

AP09566SU-N 50 µl Ask for price

Guinea pig Serum amyloid A 3 ELISA kit

E05S0229-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid A 3 ELISA kit

E05S0229-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum amyloid A 3 ELISA kit

E05S0229-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum amyloid A 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum amyloid A 3 ELISA kit

E01A42730 96T
EUR 700
Description: ELISA

Guinea pig serum albumin antibody ELISA kit

E05S0304-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig serum albumin antibody ELISA kit

E05S0304-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig serum albumin antibody ELISA kit

E05S0304-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig serum albumin antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Sterile filtered guinea pig serum, 0.2 micron

SGP30-0050 50 ml
EUR 321.36

Sterile filtered guinea pig serum, 0.2 micron

SGP30-0100 100 ml
EUR 375.96

Sterile filtered guinea pig serum, 0.2 micron

SGP30-0500 500 ml
EUR 708.24

Guinea Pig serum albumin antibody ELISA kit

E01A42787 96T
EUR 700
Description: ELISA

Innovative Grade US Origin Guinea Pig Serum

IGGPSER1000ML each
EUR 12400
Description: Innovative Grade US Origin Guinea Pig Serum

Innovative Grade US Origin Guinea Pig Serum

IGGPSER100ML each
EUR 1395
Description: Innovative Grade US Origin Guinea Pig Serum

Innovative Grade US Origin Guinea Pig Serum

IGGPSER10ML each
EUR 163
Description: Innovative Grade US Origin Guinea Pig Serum

Innovative Grade US Origin Guinea Pig Serum

IGGPSER25ML each
EUR 388
Description: Innovative Grade US Origin Guinea Pig Serum

Innovative Grade US Origin Guinea Pig Serum

IGGPSER500ML each
EUR 6588
Description: Innovative Grade US Origin Guinea Pig Serum

Innovative Grade US Origin Guinea Pig Serum

IGGPSER50ML each
EUR 737
Description: Innovative Grade US Origin Guinea Pig Serum

RLN2 guinea pig polyclonal antibody, Serum

AP02281SU-N 100 µl Ask for price

RLN2 guinea pig polyclonal antibody, Serum

AP02281SU-S 20 µl Ask for price

FSHB guinea pig polyclonal antibody, Serum

AP02297SU-N 100 µl Ask for price

FSHB guinea pig polyclonal antibody, Serum

AP02297SU-S 20 µl Ask for price

TAC1 guinea pig polyclonal antibody, Serum

EUD4501 50 µl Ask for price

PNMT guinea pig polyclonal antibody, Serum

EUD7001 50 µl Ask for price

GFAP guinea pig polyclonal antibody, Serum

BP5082 100 µl Ask for price

SND1 guinea pig polyclonal antibody, Serum

BP5102 100 µl Ask for price

CCIN guinea pig polyclonal antibody, Serum

BP5107 100 µl Ask for price

Guinea pig Serum Amyloid A(SAA) ELISA Kit

NSL0280Gp 96T
EUR 528

BFSP2 guinea pig polyclonal antibody, Serum

BP5011 100 µl Ask for price

KRT13 guinea pig polyclonal antibody, Serum

BP5076 100 µl Ask for price

KRT31 guinea pig polyclonal antibody, Serum

BP5083 100 µl Ask for price

KRT35 guinea pig polyclonal antibody, Serum

BP5086 100 µl Ask for price

KRT37 guinea pig polyclonal antibody, Serum

BP5088 100 µl Ask for price

KRT81 guinea pig polyclonal antibody, Serum

BP5090 100 µl Ask for price

KRT82 guinea pig polyclonal antibody, Serum

BP5091 100 µl Ask for price

KRT84 guinea pig polyclonal antibody, Serum

BP5093 100 µl Ask for price

KRT85 guinea pig polyclonal antibody, Serum

BP5094 100 µl Ask for price

KRT86 guinea pig polyclonal antibody, Serum

BP5095 100 µl Ask for price

KRT71 guinea pig polyclonal antibody, Serum

BP5096 100 µl Ask for price

KRT72 guinea pig polyclonal antibody, Serum

BP5097 100 µl Ask for price

KRT73 guinea pig polyclonal antibody, Serum

BP5098 100 µl Ask for price

KRT74 guinea pig polyclonal antibody, Serum

BP5099 100 µl Ask for price

KRT75 guinea pig polyclonal antibody, Serum

BP5100 100 µl Ask for price

SYNPR guinea pig polyclonal antibody, Serum

BP5104 100 µl Ask for price

CYLC2 guinea pig polyclonal antibody, Serum

BP5111 100 µl Ask for price

KRT6A guinea pig polyclonal antibody, Serum

AP32192SU-N 100 µl Ask for price

ARVCF guinea pig polyclonal antibody, Serum

AP09534SU-N 100 µl Ask for price

AHNAK guinea pig polyclonal antibody, Serum

AP09541SU-N 100 µl Ask for price

KRT25 guinea pig polyclonal antibody, Serum

AP09547SU-N 100 µl Ask for price

KRT6A guinea pig polyclonal antibody, Serum

AP09552SU-N 100 µl Ask for price

Guinea pig Serum Amyloid A2 (SAA2) ELISA Kit

abx357760-96tests 96 tests
EUR 990

ACTRT1 guinea pig polyclonal antibody, Serum

BP5105 100 µl Ask for price

ACTRT2 guinea pig polyclonal antibody, Serum

BP5106 100 µl Ask for price

HOXC13 guinea pig polyclonal antibody, Serum

BP5116 100 µl Ask for price

BTN1A1 guinea pig polyclonal antibody, Serum

AP09532SU-N 100 µl Ask for price

Guinea pig Serum Response Factor (SRF) ELISA Kit

abx357768-96tests 96 tests
EUR 990

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum response factor(SRF) ELISA kit

E05S0401-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Serum response factor(SRF) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum response factor(SRF) ELISA kit

E01A42879 96T
EUR 700
Description: ELISA

VEX Exosome Isolation Reagent (from serum)

R602 10 ml
EUR 769.2

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Serum Amyloid P Component ELISA kit

E05S0234-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig Serum Amyloid P Component in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Serum Amyloid P Component ELISA kit

E01A42735 96T
EUR 700
Description: ELISA

LOC100653049 guinea pig polyclonal antibody, Serum

BP5085 100 µl Ask for price

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 100 ML

7243150-100ML 1ML
EUR 886.99

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 500 ML

7243150-500ML 1ML
EUR 3558.97

GUINEA PIG SERUM COMPLEMENT, DOMESTIC, LIQUID, 50 ML

7243150-50ML 1ML
EUR 553.18

Guinea pig Glycosylated serum protein ELISA kit

E05G0373-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Glycosylated serum protein ELISA kit

E05G0373-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Glycosylated serum protein ELISA kit

E05G0373-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Guinea pig Glycosylated serum protein in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea Pig Glycosylated serum protein ELISA kit

E01A39754 96T
EUR 700
Description: ELISA

Insulin (INS) guinea pig polyclonal antibody, Serum

AP02014SU-N 100 µl Ask for price

Insulin (INS) guinea pig polyclonal antibody, Serum

AP02014SU-S 20 µl Ask for price

Guinea pig Serum amyloid A protein (SAA) ELISA Kit

abx357522-96tests 96 tests
EUR 990

KRT26 (397-411) guinea pig polyclonal antibody, Serum

AP09548SU-N 100 µl Ask for price

KRT27 (404-416) guinea pig polyclonal antibody, Serum

AP09549SU-N 100 µl Ask for price

KRT28 (444-457) guinea pig polyclonal antibody, Serum

AP09550SU-N 100 µl Ask for price

Cingulin (CGN) guinea pig polyclonal antibody, Serum

BP5026 100 µl Ask for price

Gastrin (GAST) guinea pig polyclonal antibody, Serum

BP5046 50 µl Ask for price
Therefore, this research develops six predictive fashions for COVID-19 analysis utilizing six totally different classifiers (i.e., BayesNet, Logistic, IBk, CR, PART, and J48) based mostly on 14 medical options. This research retrospected 114 instances from the Taizhou hospital of Zhejiang Province in China. The outcomes confirmed that the CR meta-classifier is probably the most correct classifier for predicting the constructive and detrimental COVID-19 instances with an accuracy of 84.21%. The outcomes might assist in the early analysis of COVID-19, particularly when the RT-PCR kits are usually not enough for testing the an infection and help nations, particularly the creating ones that endure from the scarcity of RT-PCR checks and specialised laboratories.

Redesigning the Genetic Polymers of Life

Redesigning the Genetic Polymers of Life
ConspectusGenomes will be seen as continually up to date reminiscence methods the place info propagated in cells is refined over time by pure choice. This course of, generally referred to as heredity and evolution, has been the sole area of DNA since the origin of prokaryotes. Now, some 3.5 billion years later, the pendulum of discovery has swung in a brand new path, with fastidiously skilled practitioners enabling the replication and evolution of “xeno-nucleic acids” or “XNAs”-synthetic genetic polymers by which the pure sugar present in DNA and RNA has been changed with a special sort of sugar moiety.
XNAs have attracted vital consideration as new polymers for artificial biology, biotechnology, and medication as a result of of their distinctive physicochemical properties which will embody elevated organic stability, enhanced chemical stability, altered helical geometry, and even elevated thermodynamics of Watson-Crick base pairing.This Account describes our contribution to the discipline of artificial biology, the place chemical synthesis and polymerase engineering have allowed my lab and others to increase the ideas of heredity and evolution to artificial genetic polymers with spine buildings which can be distinct from these present in nature.
I’ll start with a dialogue of α-l-threofuranosyl nucleic acid (TNA), a particular sort of XNA that was chosen as a mannequin system to symbolize any XNA system. I’ll then proceed to debate advances in natural chemistry that had been made to allow the synthesis of gram portions of TNA phosphoramidites and nucleoside triphosphates, the monomers used for solid-phase and polymerase-mediated TNA synthesis, respectively. Next, I’ll recount our improvement of droplet-based optical sorting (DrOPS), a single-cell microfluidic method that was established to evolve XNA polymerases in the laboratory.
This part will conclude with structural insights which were gained by fixing X-ray crystal buildings of a laboratory-evolved TNA polymerase and a pure DNA polymerase that features with basic reverse transcriptase exercise on XNA templates.The last passage of this Account will study the position that XNAs have performed in artificial biology by highlighting examples by which engineered polymerases have enabled the evolution of biologically steady affinity reagents (aptamers) and catalysts (XNAzymes) in addition to the storage and retrieval of binary info encoded in digital phrase and movie file codecs. Because these examples present solely a glimpse of what the future could have in retailer for XNA, I’ll conclude the Account with my ideas on how artificial genetic polymers might assist drive new improvements in artificial biology and molecular medication.

COVID-19 Diagnostic Testing For All – Using Non-Dilutive Saliva Sample Collection, Stabilization and Ambient Transport Devices

COVID-19 testing will not be accessible for hundreds of thousands throughout this pandemic regardless of our greatest efforts. Without enormously expanded testing of asymptomatic people, contact tracing and subsequent isolation of spreaders stays as a method for management. In an effort to extend RT-PCR assay testing for the presence of the novel beta-coronavirus SARS-CoV-2 in addition to enhance pattern assortment security, GenTegra LLC has launched two merchandise for saliva assortment and viral RNA stabilization: GTR-STM™ (GenTegra Saliva Transport Medium) and GTR-STMdk™ (GenTegra Saliva Transport Medium Direct to PCR).

Both merchandise include a proprietary formulation based mostly on GenTegra’s novel “Active Chemical Protection™” (ACP) expertise that provides non-dilutive, error-free saliva pattern assortment utilizing RNA stabilization chemical substances already dried in the assortment tube. GTR-STM can be utilized for safer saliva-based pattern assortment at house (or at a check website). Following saliva assortment, the sample-containing GTR-STM will be saved at ambient temperature throughout cargo to a licensed CLIA lab for evaluation.

SARS-CoV-2 viral RNA in GTR-STM is steady for over a month at ambient temperature, simply surviving the longest transit occasions from house to lab. GTR-STM enhances affected person consolation, comfort, compliance and reduces infectious virus publicity to important medical and lab professionals. Alternatively, the GTR-STMdk direct-into-PCR product can be utilized to enhance lab throughput and cut back reagent prices for saliva pattern assortment and testing at any lab website with entry to refrigeration. GTR-STMdk reduces lab course of time by 25% and reagent prices by 30% in comparison with different approaches.

Since GTR-STMdk retains SARS-CoV-2 viral RNA stability for 3 days at ambient temperature, it’s optimized for lab check website relatively than at house saliva assortment. SARS-COV-2 viral RNA ranges as little as 0.four genome equivalents/uL are detected in saliva samples utilizing GTR-STMdk. The elevated sensitivity of SARS-CoV-2 detection can develop COVID-19 testing to incorporate asymptomatic people utilizing pooled saliva.

Redesigning the Genetic Polymers of Life

Rapid nitrate dedication with a transportable lab-on-chip system based mostly on double microstructured assisted reactors

Determining the nitrate ranges is crucial for water high quality monitoring, and conventional strategies are restricted by excessive toxicity and low detection effectivity. Here, fast nitrate dedication was realized utilizing a transportable system based mostly on progressive three-dimensional double microstructured assisted reactors (DMARs). On-chip nitrate discount and chromogenic response had been carried out in the DMARs, and the response merchandise then flowed right into a PMMA optical detection chip for absorbance measurement. A major enhancement of response fee and effectivity was noticed in the DMARs attributable to their sizeable surface-area-to-volume ratios and hydrodynamics in the microchannels.

Different water samples had been efficiently analysed utilizing the transportable system based mostly on DMARs. The outcomes demonstrated that the system options quick detection (115 s per pattern), low reagent consumptions (26.Eight μL per pattern), significantly low consumptions of poisonous reagents (0.38 μL per pattern), good reproducibility and low relative commonplace deviations (RSDs, 0.5-1.38%). Predictably, the transportable lab-on-chip system based mostly on microstructured assisted reactors will discover extra purposes in the discipline of water high quality monitoring in the close to future.

Local remodeling of synthetic extracellular matrix microenvironments by co-cultured endometrial epithelial and stromal cells enables long-term dynamic physiological function.

Local remodeling of synthetic extracellular matrix microenvironments by co-cultured endometrial epithelial and stromal cells enables long-term dynamic physiological function.

Mucosal barrier tissues, comprising a layer of tightly-bonded epithelial cells in intimate molecular communication with an underlying matrix-rich stroma containing fibroblasts and immune cells, are outstanding targets for medication in opposition to an infection, power irritation, and different illness processes.

Although human in vitro fashions of such obstacles are wanted for mechanistic research and drug growth, variations in extracellular matrix (ECM) wants of epithelial and stromal cells hinder efforts to create such fashions.

Here, utilizing the endometrium for example mucosal barrier, we describe a synthetic, modular ECM hydrogel appropriate for 3D practical co-culture, that includes elements that may be transformed by cells and that reply dynamically to sequester native cell-secreted ECM attribute of every cell kind.

The synthetic hydrogel combines peptides with off-the-shelf reagents and is thus accessible to cell biology labs. Specifically, we first recognized a single peptide as appropriate for preliminary attachment of each endometrial epithelial and stromal cells utilizing a 2D semi-empirical display screen.

Then, utilizing a co-culture system of epithelial cells cultured on prime of gel-encapsulated stromal cells, we present that inclusion of ECM-binding peptides within the hydrogel, together with the integrin-binding peptide, results in enhanced accumulation of basement membrane beneath the epithelial layer and extra fibrillar collagen matrix meeting by stromal cells over two weeks in tradition.

Importantly, endometrial co-cultures composed of both cell traces or main cells displayed hormone-mediated differentiation as assessed by morphological adjustments and secretory protein manufacturing.

A multiplex evaluation of apical cytokine and progress issue secretion evaluating cell traces and main cells revealed strikingly completely different patterns, underscoring the significance of utilizing main cell fashions in evaluation of cell-cell communication networks. In abstract, we outline a “one-size-fits-all” synthetic ECM that enables long-term, physiologically responsive co-cultures of epithelial and stromal cells in a mucosal barrier format.

Local remodeling of synthetic extracellular matrix microenvironments by co-cultured endometrial epithelial and stromal cells enables long-term dynamic physiological function.
Local remodeling of synthetic extracellular matrix microenvironments by co-cultured endometrial epithelial and stromal cells enables long-term dynamic physiological perform.

In vivo cloning of as much as 16 kb plasmids in E. coli is so simple as PCR.

The exact meeting of outlined DNA sequences into plasmids is a vital process in bioscience analysis. While a quantity of molecular cloning methods have been developed, many strategies require specialised costly reagents or laborious experimental process.

Not surprisingly, standard cloning methods based mostly on restriction digestion and ligation are nonetheless generally utilized in routine DNA cloning. Here, we describe a easy, quick, and economical cloning methodology based mostly on RecA- and RecET-independent in vivo recombination of DNA fragments with overlapping ends utilizing E. coli.

All DNA fragments had been ready by a 2-consecutive PCR process with Q5 DNA polymerase and used immediately for transformation leading to 95% cloning accuracy and zero background from parental template plasmids. Quantitative relationships had been established between cloning effectivity and three factors-the size of overlapping nucleotides, the quantity of DNA fragments, and the dimensions of goal plasmids-which can present basic steering for choosing in vivo cloning parameters.

The methodology could also be used to precisely assemble as much as 5 DNA fragments with 25 nt overlapping ends into comparatively small plasmids, and three DNA fragments into plasmids as much as 16 kb in measurement.

The complete cloning process could also be accomplished inside 2 days by a researcher with little coaching in cloning. The mixture of excessive accuracy and zero background eliminates the necessity for screening a big quantity of colonies.

The methodology requires no enzymes apart from Q5 DNA polymerase, has no sequence restriction, is extremely dependable, and represents one of the best, quickest, and least expensive cloning methods available.

Our methodology is especially appropriate for widespread cloning duties within the lab the place the first purpose is to shortly generate a plasmid with a pre-defined sequence at low prices.